Key areas consistently high in bacteria and biofilm are the air water syringe, ultrasonic scaler and high speed hand pieces.
The conditions present in your waterlines are conducive to biofilm formation due the narrow diameter of the tubing, low water pressure, low flow rates and frequent periods of stagnation. Also, the hardeners and additives from plastic tubing may provide a nutritive source for microorganisms.
While this procedure is able to remove free-flowing organisms suspended in the waterline fluids, the beneficial effects of mechanical flushing have been shown to be transient. Recommendations from current standards/guidelines do include flushing waterlines between patients to eliminate any patient material that my be been retracted into hand pieces and air water syringes during dental procedures, however they do not [...]
Biofilms consist primarily of naturally occurring slime-producing bacteria and fungi that form microbial “communities”. This bacterial film tightly adheres and lies flat against the walls of the small-bore plastic tubing in dental units that deliver coolant water to high-speed handpieces and air/water syringes. As water flows through the microbial matrix, microorganisms may occasionally be released into the flowing water.
The pink solution in the ampoule may separate, and you may see different intensities of pink. Cold temperatures close to freezing may be a cause of this separation. Shake the vial and the solution will become a consistent pink colour again. Your test results will not be effected.
A colour change in the control vial indicates interference of a chemical in the water. The chemical is usually the waterline disinfectant/cleaner, for example chlorhexidine. If there are chemicals in your water, you can still use E|One accurately by performing an additional step. After collecting your sample, add approximately 10 mL of distilled water to the top of the [...]
A control vial will show the colour of clean water and provides a benchmark to compare the colour result of the test vial after the incubation period. Unchanged control vials also indicate that the test was performed accurately. There is no need to perform a control test every time you test your water, if your control remains unchanged in [...]
The incubator must be a dry block incubator. Temperature accelerates the result, the higher the temperature, the quicker the end result. We recommend 37˚C for 30 minutes or 60˚C for 15 minutes. Do not test at temperatures above 60˚C.
The test vial detects the presence of microorganisms (bacteria, fungi and biofilm) based on the limit of 500 cfu/mL. Microorganisms include water-borne bacteria, such as Pseudomonas species, Escherichia coli, Staphylococcus aureus, Bacillus megaterium, Pseudomonas putida, Salmonella cholerasuis, and various yeast and mold.
E-One – According to E|One directions, vial incubation should be a minimum of 30 minutes (incubated at 37°C) or a minimum of 15 minutes (incubated at 60°C). Will I have accurate results if I leave my vials in longer?
Test vials need at least the minimum time mentioned above to show accurate results for the limit of 500 cfu/mL. Highly contaminated water will show a clear, colourless result within the minimum noted times. If at these times, your test vial is still pink, you can incubate for an additional 15 or 30 minutes to ensure an accurate result. [...]